Functional expression of thyroid-stimulating hormone receptor on nano-sized bacterial magnetic particles in magnetospirillum magneticum AMB-1

Yasuhiro Sugamata, Ryo Uchiyama, Toru Honda, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino*

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

The measurement of autoantibodies to thyroid-stimulating hormone receptor (TSHR) is important for the diagnosis of autoimmune thyroid disease such as Graves' disease (GD). Although TSHR from porcine thyroid membrane is commonly used for the measurement of TSHR autoantibodies (TRAb), recombinant human TSHR (hTSHR) remains ideal in terms of stable supply and species identity. Here we set out to express recombinant hTSHR on the lipid-bilayer surface of magnetic nanoparticles from a magnetotactic bacterium, Magnetospirillum magneticum AMB-1. Using a tetracycline-inducible expression system, we successfully overexpressed functional hTSHR on bacterial magnetic particles (BacMPs) in AMB-1 via an anchor protein specific for BacMPs. The overexpressed hTSHR was membrane integrated and possessed both ligand and autoantibody binding activity. Our data suggest that hTSHR-displayed BacMPs have potential as novel tools for ligand-receptor interaction analysis or for TRAb immunoassay in GD patients.

本文言語English
ページ(範囲)14426-14438
ページ数13
ジャーナルInternational Journal of Molecular Sciences
14
7
DOI
出版ステータスPublished - 2013 7
外部発表はい

ASJC Scopus subject areas

  • コンピュータ サイエンスの応用
  • 分子生物学
  • 触媒
  • 無機化学
  • 分光学
  • 有機化学
  • 物理化学および理論化学

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