Functionalization of ultradispersed diamond for DNA detection

Jung Hoon Yang, Yoshikazu Nakano, Yasunori Murakami, Kwang Soup Song, Hiroshi Kawarada

    研究成果: Article

    7 引用 (Scopus)

    抄録

    Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

    元の言語English
    ページ(範囲)69-75
    ページ数7
    ジャーナルJournal of Nanoparticle Research
    10
    発行部数SUPPL. 1
    DOI
    出版物ステータスPublished - 2008 12

    Fingerprint

    Functionalization
    Diamond
    Strombus or kite or diamond
    Diamonds
    DNA
    deoxyribonucleic acid
    diamonds
    Nanoparticles
    Fluorescence Microscopy
    Biotechnology
    nanoparticles
    biotechnology
    Biomolecules
    Fluorescence microscopy
    immobilization
    Spectroscopy
    microscopy
    fluorescence
    Target
    spectroscopy

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics
    • Condensed Matter Physics
    • Modelling and Simulation
    • Chemistry(all)
    • Materials Science(all)
    • Bioengineering

    これを引用

    Functionalization of ultradispersed diamond for DNA detection. / Yang, Jung Hoon; Nakano, Yoshikazu; Murakami, Yasunori; Song, Kwang Soup; Kawarada, Hiroshi.

    :: Journal of Nanoparticle Research, 巻 10, 番号 SUPPL. 1, 12.2008, p. 69-75.

    研究成果: Article

    Yang, Jung Hoon ; Nakano, Yoshikazu ; Murakami, Yasunori ; Song, Kwang Soup ; Kawarada, Hiroshi. / Functionalization of ultradispersed diamond for DNA detection. :: Journal of Nanoparticle Research. 2008 ; 巻 10, 番号 SUPPL. 1. pp. 69-75.
    @article{7eb5f84aaf01486ab624e905b8ba4f88,
    title = "Functionalization of ultradispersed diamond for DNA detection",
    abstract = "Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.",
    keywords = "Amination, Carboxylation, DNA immobilization, Functionalization, Nanomedicine, Ultradispersed diamond",
    author = "Yang, {Jung Hoon} and Yoshikazu Nakano and Yasunori Murakami and Song, {Kwang Soup} and Hiroshi Kawarada",
    year = "2008",
    month = "12",
    doi = "10.1007/s11051-008-9367-2",
    language = "English",
    volume = "10",
    pages = "69--75",
    journal = "Journal of Nanoparticle Research",
    issn = "1388-0764",
    publisher = "Springer Netherlands",
    number = "SUPPL. 1",

    }

    TY - JOUR

    T1 - Functionalization of ultradispersed diamond for DNA detection

    AU - Yang, Jung Hoon

    AU - Nakano, Yoshikazu

    AU - Murakami, Yasunori

    AU - Song, Kwang Soup

    AU - Kawarada, Hiroshi

    PY - 2008/12

    Y1 - 2008/12

    N2 - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

    AB - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

    KW - Amination

    KW - Carboxylation

    KW - DNA immobilization

    KW - Functionalization

    KW - Nanomedicine

    KW - Ultradispersed diamond

    UR - http://www.scopus.com/inward/record.url?scp=54249125101&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=54249125101&partnerID=8YFLogxK

    U2 - 10.1007/s11051-008-9367-2

    DO - 10.1007/s11051-008-9367-2

    M3 - Article

    VL - 10

    SP - 69

    EP - 75

    JO - Journal of Nanoparticle Research

    JF - Journal of Nanoparticle Research

    SN - 1388-0764

    IS - SUPPL. 1

    ER -