Functionalization of ultradispersed diamond for DNA detection

Jung Hoon Yang; Yoshikazu Nakano; Yasunori Murakami; Kwang Soup Song; Hiroshi Kawarada

doi:10.1007/s11051-008-9367-2

Functionalization of ultradispersed diamond for DNA detection

Jung Hoon Yang^*, Yoshikazu Nakano, Yasunori Murakami, Kwang Soup Song, Hiroshi Kawarada

^*この研究の対応する著者

基幹理工学部

研究成果: Article › 査読

7 被引用数 (Scopus)

抄録

Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

本文言語	English
ページ（範囲）	69-75
ページ数	7
ジャーナル	Journal of Nanoparticle Research
巻	10
号	SUPPL. 1
DOI	https://doi.org/10.1007/s11051-008-9367-2
出版ステータス	Published - 2008 12月

ASJC Scopus subject areas

バイオエンジニアリング
原子分子物理学および光学
化学 (全般)
モデリングとシミュレーション
材料科学（全般）
凝縮系物理学

文献へのアクセス

10.1007/s11051-008-9367-2

他のファイルとリンク

引用スタイル

@article{7eb5f84aaf01486ab624e905b8ba4f88,

title = "Functionalization of ultradispersed diamond for DNA detection",

abstract = "Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.",

keywords = "Amination, Carboxylation, DNA immobilization, Functionalization, Nanomedicine, Ultradispersed diamond",

author = "Yang, {Jung Hoon} and Yoshikazu Nakano and Yasunori Murakami and Song, {Kwang Soup} and Hiroshi Kawarada",

note = "Funding Information: Acknowledgments This work was carried out in the Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW). This work is also supported in part by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology.",

year = "2008",

month = dec,

doi = "10.1007/s11051-008-9367-2",

language = "English",

volume = "10",

pages = "69--75",

journal = "Journal of Nanoparticle Research",

issn = "1388-0764",

publisher = "Springer Netherlands",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Functionalization of ultradispersed diamond for DNA detection

AU - Yang, Jung Hoon

AU - Nakano, Yoshikazu

AU - Murakami, Yasunori

AU - Song, Kwang Soup

AU - Kawarada, Hiroshi

N1 - Funding Information: Acknowledgments This work was carried out in the Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW). This work is also supported in part by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology.

PY - 2008/12

Y1 - 2008/12

N2 - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

AB - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

KW - Amination

KW - Carboxylation

KW - DNA immobilization

KW - Functionalization

KW - Nanomedicine

KW - Ultradispersed diamond

UR - http://www.scopus.com/inward/record.url?scp=54249125101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54249125101&partnerID=8YFLogxK

U2 - 10.1007/s11051-008-9367-2

DO - 10.1007/s11051-008-9367-2

M3 - Article

AN - SCOPUS:54249125101

VL - 10

SP - 69

EP - 75

JO - Journal of Nanoparticle Research

JF - Journal of Nanoparticle Research

SN - 1388-0764

IS - SUPPL. 1

ER -

Functionalization of ultradispersed diamond for DNA detection

抄録

ASJC Scopus subject areas

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル