Functionalized three-dimensional carbon microarrays for cancer biomarker detection

V. Penmatsa, A. R. Ruslinda, M. Beidaghi, H. Kawarada, C. Wang

研究成果: Conference contribution

8 被引用数 (Scopus)

抄録

A label-free detection strategy for the detection of platelet-derived growth factor (PDGF-BB) oncoprotein detection using signaling aptamer/protein binding complex is reported. The 3D carbon microarrays detection platform was fabricated by pyrolyzing patterned photoresist and surface functionalized using directamination technique. The detection strategy is based on the release of TOTO intercalating dye from the target binding aptamer's stem structure when it captures PDGF. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration with a very good detection limit of 5 pmol. This detection strategy is promising for the potential detection of different cancer biomarkers and proteins.

本文言語English
ホスト出版物のタイトルNano/Bio Sensors
出版社Electrochemical Society Inc.
ページ7-14
ページ数8
15
ISBN(電子版)9781623320218
ISBN(印刷版)9781623320218
DOI
出版ステータスPublished - 2012
イベントSymposium on Nano/Bio Sensors - 221st ECS Meeting - Seattle, WA, United States
継続期間: 2012 5 62012 5 10

出版物シリーズ

名前ECS Transactions
番号15
45
ISSN(印刷版)1938-5862
ISSN(電子版)1938-6737

Conference

ConferenceSymposium on Nano/Bio Sensors - 221st ECS Meeting
国/地域United States
CitySeattle, WA
Period12/5/612/5/10

ASJC Scopus subject areas

  • 工学(全般)

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