GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination

Yoshimasa Takizawa, Yong Qing, Motoki Takaku, Takako Ishida, Yuichi Morozumi, Takashi Tsujita, Toshiaki Kogame, Kouji Hirota, Masayuki Takahashi, Takehiko Shibata, Hitoshi Kurumizaka, Shunichi Takeda

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    RAD51 is a key factor in homologous recombination (HR) and plays an essential role in cellular proliferation by repairing DNA damage during replication. The assembly of RAD51 at DNA damage is strictly controlled by RAD51 mediators, including BRCA1 and BRCA2 found that human RAD51 directly binds GEMIN2/SIP1, a protein involved in spliceosome biogenesis. Biochemical analyses indicated that GEMIN2 enhances the RAD51-DNA complex formation by inhibiting RAD51 dissociation from DNA, and thereby stimulates RAD51-mediated homologous pairing. GEMIN2 also enhanced the RAD51-mediated strand exchange, when RPA was pre-bound to ssDNA before the addition of RAD51 analyze the function of GEMIN2, we depleted GEMIN2 in the chicken DT40 line and in human cells. The loss of GEMIN2 reduced HR efficiency and resulted in a significant decrease in the number of RAD51 subnuclear foci, as observed in cells deficient in BRCA1 and BRCA2. These observations and our biochemical analyses reveal that GEMIN2 regulates HR as a novel RAD51 mediator.

    元の言語English
    記事番号gkq271
    ページ(範囲)5059-5074
    ページ数16
    ジャーナルNucleic Acids Research
    38
    発行部数15
    DOI
    出版物ステータスPublished - 2010 4 19

    ASJC Scopus subject areas

    • Genetics

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    Takizawa, Y., Qing, Y., Takaku, M., Ishida, T., Morozumi, Y., Tsujita, T., Kogame, T., Hirota, K., Takahashi, M., Shibata, T., Kurumizaka, H., & Takeda, S. (2010). GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids Research, 38(15), 5059-5074. [gkq271]. https://doi.org/10.1093/nar/gkq271