Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100

Takuya Imamura, Naka Hattori, Kunio Shiota*, Tomoya Ogawa

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.

本文言語English
ページ(範囲)534-539
ページ数6
ジャーナルJournal of Biochemistry
124
3
出版ステータスPublished - 1998 9
外部発表はい

ASJC Scopus subject areas

  • 生化学

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