Generation and characterization of monoclonal antibodies against multiple epitopes on the C‐terminal half of envelope gp46 of human T‐cell leukemia virus type‐I (HTLV‐I)

Yuetsu Tanaka*, Masazumi Yasumoto, Hiroshi Nyunoya, Tsutomu Ogura, Masayoshi Kikuchi, Kunitada Shimotohno, Hiroshi Shiraki, Naotaka Kuroda, Hisatoshi Shida, Hideki Tozawa

*この研究の対応する著者

研究成果: Article査読

36 被引用数 (Scopus)

抄録

In order to study the antigenicity of envelope 46 kDa glycoprotein (gp46) of human T‐cell leukemia virus type‐l (HTLV‐I), we have generated monoclonal anti‐gp46 antibodies (MAbs), REY‐7, REY‐11, REY‐16, REY‐30, MET‐2 and MET‐3 from rats and mice. Immunoblot and immunofluorescence assays showed that these MAbs recognize gp46 and its related antigens, and specifically stained HTLV‐I‐bearing cells. All MAbs reacted with a recombinant gp46 antigen, NI47, expressing the 147 amino acids in the C‐terminal half of gp46. By using various synthetic peptides corresponding to the gp46 sequence, epitopes recognized by REY‐7 and MET‐3, REY‐11 and RE Y‐16, and REY‐30 were mapped to regions corresponding to the amino acids 175‐199, 253‐282 and 288‐312, respectively. MET‐2 did not react with any of the peptides used. These results indicate that the present MAbs are directed against at least 4 distinct epitopes expressed on the C‐terminal half of gp46. The binding of these MAbs to gp46 was specifically inhibited by sera from HTLV‐I‐infected individuals, but none of these MAbs inhibited the cell fusion activity of HTLV‐I.

本文言語English
ページ(範囲)675-681
ページ数7
ジャーナルInternational Journal of Cancer
46
4
DOI
出版ステータスPublished - 1990 10月 15
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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