Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostellium

Akira Nagasaki, Eugenio L. de Hostos, Taro Q.P. Uyeda*

*この研究の対応する著者

研究成果: Article査読

59 被引用数 (Scopus)

抄録

Myosin-II-null cells of Dictyostelium discoideum cannot divide in suspension, consistent with the dogma that myosin II drives constriction of the cleavage furrow and, consequently, cytokinesis (cytokinesis A). Nonetheless, when grown on substrates, these cells exhibit efficient, cell-cycle-coupled division, suggesting that they possess a novel, myosin-II-independent, adhesion-dependent method of cytokinesis (cytokinesis B). Here we show that double mutants lacking myosin II and either AmiA or coronin, both of which are implicated in cytokinesis B, are incapable of cell-cycle-coupled cytokinesis. These double mutants multiplied mainly by cytokinesis C, a third, inefficient, method of cell division, which requires substrate adhesion and is independent of cell cycle progression. In contrast, double mutants lacking AmiA and coronin were no sicker than each of the single mutants, indicating that the severe defects of myosin II-/AmiA- or myosin II-/coronin- mutants are not simple additive effects of two mutations. We take this as genetic evidence for two parallel pathways both of which lead to cell-cycle-coupled cytokinesis. This conclusion is supported by differences in morphological changes during cytokinesis in the mutant cell lines.

本文言語English
ページ(範囲)2241-2251
ページ数11
ジャーナルJournal of Cell Science
115
10
出版ステータスPublished - 2002 5月 15
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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