Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint

Yuko Sukegawa, Takahiro Negishi, Yo Kikuchi, Keiko Ishii, Miyuki Imanari, Farzan Ghanegolmohammadi, Satoru Nogami, Yoshikazu Ohya

研究成果: Article

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The cell wall integrity checkpoint monitors synthesis of cell wall materials during the Saccharomyces cerevisiae cell cycle. Upon perturbation of cell wall synthesis, the cell wall integrity checkpoint is activated, downregulating Clb2 transcription. Here, we identified genes involved in this checkpoint by genetic screening of deletion mutants. In addition to the previously identified dynactin complex, the Las17 complex, in particular the Bzz1 and Vrp1 components, plays a role in this checkpoint. We also revealed that the high osmolarity glycerol (HOG) and cell wall integrity mitogen-activated protein kinase (MAPK) signaling pathways are essential for checkpoint function. The defective checkpoint caused by the deficient dynactin and Las17 complexes was rescued by hyperactivation of the cell wall integrity MAPK pathway, but not by the activated form of Hog1, suggesting an order to these signaling pathways. Mutation of Fkh2, a transcription factor important for Clb2 expression, suppressed the checkpoint-defective phenotype of Las17, HOG MAPK and cell wall integrity MAPK mutations. These results provide genetic evidence that signaling from the cell surface regulates the downstream transcriptional machinery to activate the cell wall integrity checkpoint.

元の言語English
記事番号jcs219063
ジャーナルJournal of Cell Science
131
発行部数13
DOI
出版物ステータスPublished - 2018 7 1
外部発表Yes

ASJC Scopus subject areas

  • Cell Biology

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    Sukegawa, Y., Negishi, T., Kikuchi, Y., Ishii, K., Imanari, M., Ghanegolmohammadi, F., Nogami, S., & Ohya, Y. (2018). Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint. Journal of Cell Science, 131(13), [jcs219063]. https://doi.org/10.1242/jcs.219063