Genetic engineering in marine cyanobacteria

Tadashi Matsunaga, Haruko Takeyama

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Many species of microalgae producing useful materials have been isolated from marine environments. For their industrial application, widely applicable and stable gene expression is required. It is necessary to establish gene transfer methods as an essential first step in genetic manipulation. Although gene transfer techniques for cyanobacteria have been developed, only naturally transformable strains have been used. Here, we describe recent progress made in developing gene transfer methods for marine cyanobacteria. The following are covered: (1) transformation, (2) electroporation, (3) conjugation, (4) particle gun. A plasmid from the marine cyanobacterium, Synechococcus sp., whose copy number is dependent on salinity, was characterized. This plasmid is being used to develop a stable and controllable gene expression system.

本文言語English
ページ(範囲)77-84
ページ数8
ジャーナルJournal of Applied Phycology
7
1
DOI
出版ステータスPublished - 1995 2 1
外部発表はい

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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