Genetic engineering of a Ca2+ dependent chemical switch into the linear biomotor kinesin

Kaoru Konishi, Taro Q.P. Uyeda*, Tai Kubo

*この研究の対応する著者

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Kinesin is a linear motor protein driven by energy released by ATP hydrolysis. In the present work, we genetically installed an M13 peptide sequence into Loop 12 of kinesin, which is one of the major microtubule binding regions of the protein. Because the M13 sequence has high affinity for Ca2+-calmodulin, the association of the engineered kinesin with microtubules showed a steep Ca2+-dependency in ATPase activity at Ca2+ concentrations of pCa 6.5-8. The calmodulin-binding domain of plant kinesin-like calmodulin-binding protein is also known to confer Ca2+-calmodulin regulation to kinesins. Unlike this plant kinesin, however, our novel engineered kinesin achieves this regulation while maintaining the interaction between kinesin and microtubules. The engineered kinesin is switched on/off reversibly by an external signal (i.e., Ca2+-calmodulin) and, thus, can be used as a model system for a bio/nano-actuator.

本文言語English
ページ(範囲)3589-3594
ページ数6
ジャーナルFEBS Letters
580
15
DOI
出版ステータスPublished - 2006 6月 26
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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