Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae

Satoshi Yoshida, Yoshikazu Ohya, Akihiko Nakano, Yasuhiro Anraku

研究成果: Article

72 引用 (Scopus)

抄録

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

元の言語English
ページ(範囲)631-640
ページ数10
ジャーナルMGG Molecular & General Genetics
242
発行部数6
DOI
出版物ステータスPublished - 1994 3 1
外部発表Yes

Fingerprint

Saccharomyces cerevisiae
Protein Kinase C
Mutation
Genes
Phenotype
1-Phosphatidylinositol 4-Kinase
Fungal Proteins
Staurosporine
Protein Kinases
Molecular Biology
Cell Cycle
Amino Acids
Temperature
Growth
Proteins

ASJC Scopus subject areas

  • Genetics

これを引用

Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae. / Yoshida, Satoshi; Ohya, Yoshikazu; Nakano, Akihiko; Anraku, Yasuhiro.

:: MGG Molecular & General Genetics, 巻 242, 番号 6, 01.03.1994, p. 631-640.

研究成果: Article

Yoshida, Satoshi ; Ohya, Yoshikazu ; Nakano, Akihiko ; Anraku, Yasuhiro. / Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae. :: MGG Molecular & General Genetics. 1994 ; 巻 242, 番号 6. pp. 631-640.
@article{a9c5167f87544021821ac87bf5286ad6,
title = "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae",
abstract = "Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.",
keywords = "Cell cycle, Cell lysis, Phosphatidylinositol 4-kinase, Protein kinase C, Staurosporine",
author = "Satoshi Yoshida and Yoshikazu Ohya and Akihiko Nakano and Yasuhiro Anraku",
year = "1994",
month = "3",
day = "1",
doi = "10.1007/BF00283416",
language = "English",
volume = "242",
pages = "631--640",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae

AU - Yoshida, Satoshi

AU - Ohya, Yoshikazu

AU - Nakano, Akihiko

AU - Anraku, Yasuhiro

PY - 1994/3/1

Y1 - 1994/3/1

N2 - Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

AB - Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

KW - Cell cycle

KW - Cell lysis

KW - Phosphatidylinositol 4-kinase

KW - Protein kinase C

KW - Staurosporine

UR - http://www.scopus.com/inward/record.url?scp=0028218165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028218165&partnerID=8YFLogxK

U2 - 10.1007/BF00283416

DO - 10.1007/BF00283416

M3 - Article

C2 - 8152413

AN - SCOPUS:0028218165

VL - 242

SP - 631

EP - 640

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 6

ER -