Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS

Shuji Shigenobu, Hidemi Watanabe, Masahira Hattori, Yoshiyuki Sakaki*, Hajime Ishikawa

*この研究の対応する著者

研究成果: Article査読

1032 被引用数 (Scopus)

抄録

Almost all aphid species (Homoptera, Insecta) have 60-80 huge cells called bacteriocytes, within which are round-shaped bacteria that are designated Buchnera. These bacteria are maternally transmitted to eggs and embryos through host generations, and the mutualism between the host and the bacteria is so obligate that neither can reproduce independently. Buchnera is a close relative of Escherichia coli, but it contains more than 100 genomic copies per cell, and its genome size is only a seventh of that of E. coli. Here we report the complete genome sequence of Buchnera sp. strain APS, which is composed of one 640,681-base-pair chromosome and two small plasmids. There are genes for the biosyntheses of amino acids essential for the hosts in the genome, but those for non-essential amino acids are missing, indicating complementarity and syntrophy between the host and the symbiont. In addition, Buchnera lacks genes for the biosynthesis of cell-surface components, including lipopolysaccharides and phospholipids, regulator genes and genes involved in defence of the cell. These results indicate that Buchnera is completely symbiotic and viable only in its limited niche, the bacteriocyte.

本文言語English
ページ(範囲)81-86
ページ数6
ジャーナルNature
407
6800
DOI
出版ステータスPublished - 2000 9月
外部発表はい

ASJC Scopus subject areas

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