Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350

Yasuo Ohnishi, Jun Ishikawa, Hirofumi Hara, Hirokazu Suzuki, Miwa Ikenoya, Haruo Ikeda, Atsushi Yamashita, Masahira Hattori, Sueharu Horinouchi

研究成果: Article

384 引用 (Scopus)

抄録

We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5′-GGA-3′, are different from those of typical telomeres conserved among other Streptomyces species. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species, Streptomyces coelicolor A3(2) and Streptomyces avermitilis, clarified not only the characteristics of the S. griseus genome but also the existence of 24 Streptomyces-specific proteins. The S. griseus genome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a γ-butyrolactone signaling molecule) regulatory cascade in S. griseus.

元の言語English
ページ(範囲)4050-4060
ページ数11
ジャーナルJournal of Bacteriology
190
発行部数11
DOI
出版物ステータスPublished - 2008 6
外部発表Yes

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Streptomyces griseus
Streptomycin
Telomere
Genome
Streptomyces
Terminal Repeat Sequences
Multigene Family
Proteins
Base Pairing
rRNA Operon
Streptomyces coelicolor
Secondary Metabolism
Chromosomes, Human, Pair 8
Pseudogenes
Base Composition
Aminoglycosides
Gene Expression Profiling
Transfer RNA
Oligonucleotide Array Sequence Analysis
Morphogenesis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

これを引用

Ohnishi, Y., Ishikawa, J., Hara, H., Suzuki, H., Ikenoya, M., Ikeda, H., ... Horinouchi, S. (2008). Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. Journal of Bacteriology, 190(11), 4050-4060. https://doi.org/10.1128/JB.00204-08

Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. / Ohnishi, Yasuo; Ishikawa, Jun; Hara, Hirofumi; Suzuki, Hirokazu; Ikenoya, Miwa; Ikeda, Haruo; Yamashita, Atsushi; Hattori, Masahira; Horinouchi, Sueharu.

:: Journal of Bacteriology, 巻 190, 番号 11, 06.2008, p. 4050-4060.

研究成果: Article

Ohnishi, Y, Ishikawa, J, Hara, H, Suzuki, H, Ikenoya, M, Ikeda, H, Yamashita, A, Hattori, M & Horinouchi, S 2008, 'Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350', Journal of Bacteriology, 巻. 190, 番号 11, pp. 4050-4060. https://doi.org/10.1128/JB.00204-08
Ohnishi Y, Ishikawa J, Hara H, Suzuki H, Ikenoya M, Ikeda H その他. Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. Journal of Bacteriology. 2008 6;190(11):4050-4060. https://doi.org/10.1128/JB.00204-08
Ohnishi, Yasuo ; Ishikawa, Jun ; Hara, Hirofumi ; Suzuki, Hirokazu ; Ikenoya, Miwa ; Ikeda, Haruo ; Yamashita, Atsushi ; Hattori, Masahira ; Horinouchi, Sueharu. / Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. :: Journal of Bacteriology. 2008 ; 巻 190, 番号 11. pp. 4050-4060.
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abstract = "We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2{\%}, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5′-GGA-3′, are different from those of typical telomeres conserved among other Streptomyces species. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species, Streptomyces coelicolor A3(2) and Streptomyces avermitilis, clarified not only the characteristics of the S. griseus genome but also the existence of 24 Streptomyces-specific proteins. The S. griseus genome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a γ-butyrolactone signaling molecule) regulatory cascade in S. griseus.",
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