Genome-wide profiling of DNA methylation in human cancer cells

Katsumi Ogoshi*, Shin ichi Hashimoto, Yoichiro Nakatani, Wei Qu, Kenshiro Oshima, Katsushi Tokunaga, Sumio Sugano, Masahira Hattori, Shinichi Morishita, Kouji Matsushima

*この研究の対応する著者

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Global changes in DNA methylation correlate with altered gene expression and genomic instability in cancer. We have developed a methylation-specific digital sequencing (MSDS) method that can assess DNA methylation on a genomic scale. MSDS is a simple, low-cost method that combines the use of methylation-sensitive restriction enzymes with second generation sequencing technology. DNA methylation in two colon cancer cell lines, HT29 and HCT116, was measured using MSDS. When methylation levels were compared between the two cell lines, many differentially methylated regions (DMRs) were identified in CpG island shore regions (located within 2 kb of a CpG island), gene body regions and intergenic regions. The number of DMRs in the vicinity of gene transcription start sites correlated with the level of expression of TACC1, CLDN1, and PLEKHC1 (FERMT2) genes, which have been linked to carcinogenesis. The MSDS method has the potential to provide novel insight into the functional complexity of the human genome.

本文言語English
ページ(範囲)280-287
ページ数8
ジャーナルGenomics
98
4
DOI
出版ステータスPublished - 2011 10
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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