Genomic structure, expression, and transcriptional regulation of human Gal β1,3 GalNAc α2,3-sialyltransferase gene

Akiyoshi Taniguchi*, Takashi Morishima, Yuna Tsujita, Yoshiko Matsumoto, Kojiro Matsumoto

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

In this report, we describe transcriptional regulation of the human Gal β1,3 GalNAc α2,3-sialyltransferase II (hST3Gal II) gene. The results of 5-RACE showed that the forms of two mRNAs differed only in the 5-untranslated region (Types 1 and 2). According to analysis of the genomic structure, the transcriptional regulation of Type 1 and Type 2 mRNA isoforms depended on the p1 and p2 promoters, respectively. Both the mRNA isoforms were detected in various human tissues except colon, skeletal muscle, and peripheral blood leukocytes by RT-PCR analysis. In colon tissue, the Type 2 mRNA was detected, however, Type 1 mRNA was not detected. To elucidate the molecular basis of hST3Gal II gene expression, we isolated and characterized the function of the genomic region of hST3Gal II containing the p1 and p2 promoters. The activity of p2 promoter is much higher than that of the p1 promoter in the colon adenocarcinoma cell line, COLO205. These results suggest that the hST3Gal II gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of transcription.

本文言語English
ページ(範囲)570-576
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
300
2
DOI
出版ステータスPublished - 2003 1 10
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Genomic structure, expression, and transcriptional regulation of human Gal β1,3 GalNAc α2,3-sialyltransferase gene」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル