Glutamatergic neurotransmission in the procerebrum (Olfactory Center) of a terrestrial mollusk

Ryota Matsuo*, Suguru Kobayashi, Satoshi Watanabe, Shigeyuki Namiki, Sho Iinuma, Hirokazu Sakamoto, Kenzo Hirose, Etsuro Ito

*この研究の対応する著者

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The terrestrial slug Limax has the ability to learn odor associations. This ability depends on the function of the procerebrum, the secondary olfactory center in the brain. Among the various neurotransmitters that are thought to be involved in the function of the procerebrum, glutamate is one of the most important molecules. However, the existence and function of glutamate in this system have been proposed solely on the basis of a few lines of indirect evidence from pharmacological experiments. In the present study, we demonstrated the existence and release of glutamate as a neurotransmitter in the procerebrum of Limax, by using three different techniques: 1) immunohistochemistry of glutamate, 2) in situ hybridization to mRNA of the vesicular glutamate transporter, and 3) real-time imaging of glutamate release within the procerebrum using the glutamate optical sensor EOS2. The release of glutamate within the cell mass layer of the procerebrum was synchronized with oscillation of the local field potential and had the same physiological properties as this oscillation; both were blocked by a serotonin antagonist and were propagated in an apical to basal direction in the procerebrum. Our observations suggest strongly that the oscillation of the local field potential is driven by the glutamate released by bursting neurons in the procerebrum.

本文言語English
ページ(範囲)3011-3023
ページ数13
ジャーナルJournal of Neuroscience Research
87
13
DOI
出版ステータスPublished - 2009 10月 1
外部発表はい

ASJC Scopus subject areas

  • 細胞および分子神経科学

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