Glycosphingolipid composition of rat placenta: Changes associated with stage of pregnancy

S. Itonori, T. Shirai, Y. Kiso, Y. Ohashi, K. Shiota, T. Ogawa

研究成果: Article

12 引用 (Scopus)

抄録

The composition of glycolipids and their changes in the placenta were investigated in the normal pregnant rat. Total lipid fractions extracted from the placenta between days 12 and 20 of pregnancy (day 0 = oestrus) were subjected to glycolipid analysis using DEAE-Sephadex chromatography, silica-gel HPLC, silica-gel TLC, TLC/immunostaining, matrix-assisted secondary-ion mass spectrometry in the negative-ion mode and 1H NMR. Glycolipids identified in the rat placenta were: gangliosides G(M3) (NeuAcLacCer and NeuGcLacCer) and G(D3) (NeuAcNeuAcLacCer, NeuAcNeuGcLacCer and NeuGcNeuAcLacCer), and neutral glycolipids ceramide monosaccharide (CMH) (GlcCer), ceramide disaccharide (CDH) (LacCer), ceramide trisaccharide (CTH) (Gb3Cer) and ceramide tetrasaccharide (CQH) (Gb4Cer). The content of neutral glycolipids was higher than that of gangliosides throughout pregnancy. Of the neutral glycolipids, CMH and CTH predominated and the level of CDH was low at mid-pregnancy. During late pregnancy, CMH and CTH decreased and CDH increased markedly. CQH remained at a low level throughout pregnancy. Of the gangliosides, G(M3) was predominant on days 12-16 and then decreased, whereas G(D3) which was low on day 12, increased slightly on day 16 and maintained the same level thereafter. Immunohistochemical studies indicated that these changes in the expression of major gangliosides from G(M3) to G(D3) occurred in labyrinthine trophoblasts. Thus expression of these glycolipids appears to change markedly during pregnancy.

元の言語English
ページ(範囲)399-405
ページ数7
ジャーナルBiochemical Journal
307
発行部数2
出版物ステータスPublished - 1995
外部発表Yes

Fingerprint

Glycosphingolipids
Ceramides
Placenta
Rats
Glycolipids
Pregnancy
Chemical analysis
G(M3) Ganglioside
Trisaccharides
Monosaccharides
Disaccharides
Silica Gel
Secondary Ion Mass Spectrometry
DEAE-Dextran
Gangliosides
Estrus
Trophoblasts
Secondary ion mass spectrometry
Chromatography
Negative ions

ASJC Scopus subject areas

  • Biochemistry

これを引用

Itonori, S., Shirai, T., Kiso, Y., Ohashi, Y., Shiota, K., & Ogawa, T. (1995). Glycosphingolipid composition of rat placenta: Changes associated with stage of pregnancy. Biochemical Journal, 307(2), 399-405.

Glycosphingolipid composition of rat placenta : Changes associated with stage of pregnancy. / Itonori, S.; Shirai, T.; Kiso, Y.; Ohashi, Y.; Shiota, K.; Ogawa, T.

:: Biochemical Journal, 巻 307, 番号 2, 1995, p. 399-405.

研究成果: Article

Itonori, S, Shirai, T, Kiso, Y, Ohashi, Y, Shiota, K & Ogawa, T 1995, 'Glycosphingolipid composition of rat placenta: Changes associated with stage of pregnancy', Biochemical Journal, 巻. 307, 番号 2, pp. 399-405.
Itonori S, Shirai T, Kiso Y, Ohashi Y, Shiota K, Ogawa T. Glycosphingolipid composition of rat placenta: Changes associated with stage of pregnancy. Biochemical Journal. 1995;307(2):399-405.
Itonori, S. ; Shirai, T. ; Kiso, Y. ; Ohashi, Y. ; Shiota, K. ; Ogawa, T. / Glycosphingolipid composition of rat placenta : Changes associated with stage of pregnancy. :: Biochemical Journal. 1995 ; 巻 307, 番号 2. pp. 399-405.
@article{5dd5b9be2497423e8bd40256ae9c1c13,
title = "Glycosphingolipid composition of rat placenta: Changes associated with stage of pregnancy",
abstract = "The composition of glycolipids and their changes in the placenta were investigated in the normal pregnant rat. Total lipid fractions extracted from the placenta between days 12 and 20 of pregnancy (day 0 = oestrus) were subjected to glycolipid analysis using DEAE-Sephadex chromatography, silica-gel HPLC, silica-gel TLC, TLC/immunostaining, matrix-assisted secondary-ion mass spectrometry in the negative-ion mode and 1H NMR. Glycolipids identified in the rat placenta were: gangliosides G(M3) (NeuAcLacCer and NeuGcLacCer) and G(D3) (NeuAcNeuAcLacCer, NeuAcNeuGcLacCer and NeuGcNeuAcLacCer), and neutral glycolipids ceramide monosaccharide (CMH) (GlcCer), ceramide disaccharide (CDH) (LacCer), ceramide trisaccharide (CTH) (Gb3Cer) and ceramide tetrasaccharide (CQH) (Gb4Cer). The content of neutral glycolipids was higher than that of gangliosides throughout pregnancy. Of the neutral glycolipids, CMH and CTH predominated and the level of CDH was low at mid-pregnancy. During late pregnancy, CMH and CTH decreased and CDH increased markedly. CQH remained at a low level throughout pregnancy. Of the gangliosides, G(M3) was predominant on days 12-16 and then decreased, whereas G(D3) which was low on day 12, increased slightly on day 16 and maintained the same level thereafter. Immunohistochemical studies indicated that these changes in the expression of major gangliosides from G(M3) to G(D3) occurred in labyrinthine trophoblasts. Thus expression of these glycolipids appears to change markedly during pregnancy.",
author = "S. Itonori and T. Shirai and Y. Kiso and Y. Ohashi and K. Shiota and T. Ogawa",
year = "1995",
language = "English",
volume = "307",
pages = "399--405",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Glycosphingolipid composition of rat placenta

T2 - Changes associated with stage of pregnancy

AU - Itonori, S.

AU - Shirai, T.

AU - Kiso, Y.

AU - Ohashi, Y.

AU - Shiota, K.

AU - Ogawa, T.

PY - 1995

Y1 - 1995

N2 - The composition of glycolipids and their changes in the placenta were investigated in the normal pregnant rat. Total lipid fractions extracted from the placenta between days 12 and 20 of pregnancy (day 0 = oestrus) were subjected to glycolipid analysis using DEAE-Sephadex chromatography, silica-gel HPLC, silica-gel TLC, TLC/immunostaining, matrix-assisted secondary-ion mass spectrometry in the negative-ion mode and 1H NMR. Glycolipids identified in the rat placenta were: gangliosides G(M3) (NeuAcLacCer and NeuGcLacCer) and G(D3) (NeuAcNeuAcLacCer, NeuAcNeuGcLacCer and NeuGcNeuAcLacCer), and neutral glycolipids ceramide monosaccharide (CMH) (GlcCer), ceramide disaccharide (CDH) (LacCer), ceramide trisaccharide (CTH) (Gb3Cer) and ceramide tetrasaccharide (CQH) (Gb4Cer). The content of neutral glycolipids was higher than that of gangliosides throughout pregnancy. Of the neutral glycolipids, CMH and CTH predominated and the level of CDH was low at mid-pregnancy. During late pregnancy, CMH and CTH decreased and CDH increased markedly. CQH remained at a low level throughout pregnancy. Of the gangliosides, G(M3) was predominant on days 12-16 and then decreased, whereas G(D3) which was low on day 12, increased slightly on day 16 and maintained the same level thereafter. Immunohistochemical studies indicated that these changes in the expression of major gangliosides from G(M3) to G(D3) occurred in labyrinthine trophoblasts. Thus expression of these glycolipids appears to change markedly during pregnancy.

AB - The composition of glycolipids and their changes in the placenta were investigated in the normal pregnant rat. Total lipid fractions extracted from the placenta between days 12 and 20 of pregnancy (day 0 = oestrus) were subjected to glycolipid analysis using DEAE-Sephadex chromatography, silica-gel HPLC, silica-gel TLC, TLC/immunostaining, matrix-assisted secondary-ion mass spectrometry in the negative-ion mode and 1H NMR. Glycolipids identified in the rat placenta were: gangliosides G(M3) (NeuAcLacCer and NeuGcLacCer) and G(D3) (NeuAcNeuAcLacCer, NeuAcNeuGcLacCer and NeuGcNeuAcLacCer), and neutral glycolipids ceramide monosaccharide (CMH) (GlcCer), ceramide disaccharide (CDH) (LacCer), ceramide trisaccharide (CTH) (Gb3Cer) and ceramide tetrasaccharide (CQH) (Gb4Cer). The content of neutral glycolipids was higher than that of gangliosides throughout pregnancy. Of the neutral glycolipids, CMH and CTH predominated and the level of CDH was low at mid-pregnancy. During late pregnancy, CMH and CTH decreased and CDH increased markedly. CQH remained at a low level throughout pregnancy. Of the gangliosides, G(M3) was predominant on days 12-16 and then decreased, whereas G(D3) which was low on day 12, increased slightly on day 16 and maintained the same level thereafter. Immunohistochemical studies indicated that these changes in the expression of major gangliosides from G(M3) to G(D3) occurred in labyrinthine trophoblasts. Thus expression of these glycolipids appears to change markedly during pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=0028914310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028914310&partnerID=8YFLogxK

M3 - Article

C2 - 7733875

AN - SCOPUS:0028914310

VL - 307

SP - 399

EP - 405

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 2

ER -