Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system

Daxiang Cui*, Hong Zhang, Kan Wang, Feng Gao, Xueqing Zhang, Toru Asahi, Rong He, Tetsuya Osaka

*この研究の対応する著者

研究成果査読

7 被引用数 (Scopus)

抄録

Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

本文言語English
ページ(範囲)120-125
ページ数6
ジャーナルNano Biomedicine and Engineering
3
2
DOI
出版ステータスPublished - 2011

ASJC Scopus subject areas

  • 生体医工学

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