TY - JOUR
T1 - Gold Nanoshell-Mediated Remote Myotube Activation
AU - Marino, Attilio
AU - Arai, Satoshi
AU - Hou, Yanyan
AU - Degl'Innocenti, Andrea
AU - Cappello, Valentina
AU - Mazzolai, Barbara
AU - Chang, Young Tae
AU - Mattoli, Virgilio
AU - Suzuki, Madoka
AU - Ciofani, Gianni
PY - 2017/3/28
Y1 - 2017/3/28
N2 - Mild heat stimulation of muscle cells within the physiological range represents an intriguing approach for the modulation of their functions. In this work, photothermal conversion was exploited to remotely stimulate striated muscle cells by using gold nanoshells (NSs) in combination with near-infrared (NIR) radiation. Temperature increments of approximately 5 °C were recorded by using an intracellular fluorescent molecular thermometer and were demonstrated to efficiently induce myotube contraction. The mechanism at the base of this phenomenon was thoroughly investigated and was observed to be a Ca2+-independent event directly involving actin-myosin interactions. Finally, chronic remote photothermal stimulations significantly increased the mRNA transcription of genes encoding heat shock proteins and sirtuin 1, a protein which in turn can induce mitochondrial biogenesis. Overall, we provide evidence that remote NIR + NS muscle excitation represents an effective wireless stimulation technique with great potential in the fields of muscle tissue engineering, regenerative medicine, and bionics.
AB - Mild heat stimulation of muscle cells within the physiological range represents an intriguing approach for the modulation of their functions. In this work, photothermal conversion was exploited to remotely stimulate striated muscle cells by using gold nanoshells (NSs) in combination with near-infrared (NIR) radiation. Temperature increments of approximately 5 °C were recorded by using an intracellular fluorescent molecular thermometer and were demonstrated to efficiently induce myotube contraction. The mechanism at the base of this phenomenon was thoroughly investigated and was observed to be a Ca2+-independent event directly involving actin-myosin interactions. Finally, chronic remote photothermal stimulations significantly increased the mRNA transcription of genes encoding heat shock proteins and sirtuin 1, a protein which in turn can induce mitochondrial biogenesis. Overall, we provide evidence that remote NIR + NS muscle excitation represents an effective wireless stimulation technique with great potential in the fields of muscle tissue engineering, regenerative medicine, and bionics.
KW - C2C12 myotubes
KW - calcium imaging
KW - cell heating
KW - intracellular thermometry
KW - near-infrared radiation
UR - http://www.scopus.com/inward/record.url?scp=85016414430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016414430&partnerID=8YFLogxK
U2 - 10.1021/acsnano.6b08202
DO - 10.1021/acsnano.6b08202
M3 - Article
C2 - 28107625
AN - SCOPUS:85016414430
VL - 11
SP - 2494
EP - 2505
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 3
ER -