Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells

Guan Yu Chen, Zeyang Li, Christopher S. Theile, Neelkanth M. Bardhan, Priyank V. Kumar, Joao N. Duarte, Takeshi Maruyama, Ali Rashidfarrokh, Angela M. Belcher, Hidde L. Ploegh*

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Peripheral blood can provide valuable information on an individual's immune status. Cell-based assays typically target leukocytes and their products. Characterization of leukocytes from whole blood requires their separation from the far more numerous red blood cells.1 Current methods to classify leukocytes, such as recovery on antibody-coated beads or fluorescence-activated cell sorting require long sample preparation times and relatively large sample volumes.2 A simple method that enables the characterization of cells from a small peripheral whole blood sample could overcome limitations of current analytical techniques. We describe the development of a simple graphene oxide surface coated with single-domain antibody fragments. This format allows quick and efficient capture of distinct WBC subpopulations from small samples (∼30 μL) of whole blood in a geometry that does not require any specialized equipment such as cell sorters or microfluidic devices.

本文言語English
ページ(範囲)17178-17183
ページ数6
ジャーナルChemistry - A European Journal
21
48
DOI
出版ステータスPublished - 2015 11 23
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 有機化学

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