Guide DNA technique reveals that the protein component of bacterial ribonuclease P is a modifier for substrate recognition

Terumichi Tanaka*, Hideo Baba, Yoshiaki Hori, Yo Kikuchi

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We developed a guide DNA technique with which the cleavage efficiency of pre-tRNA substrate raised in the RNase P reaction. The 20-mer guide DNAs hybridizing to the upstream region of the cleaving site enhanced the cleavage reactions of RNA substrates by Escherichia coli RNase P. This guide DNA technique was also applicable to cleavage site selection by choosing the DNA-hybridizing site. Results showed that RNase P accepts DNA/RNA double-stranded 5′-leader region with high catalytic efficiency as well as single-stranded RNA region in pre-tRNAs as substrates, which suggests that the protein component of bacterial RNase P prefers bulky nucleotides. The protein component did not affect the normal 5′-processing reaction of pre-tRNAs, but enhanced the mis-cleaving (hyperprocessing) reactions of tRNA in non-cloverleaf folding. Our results suggested that the protein component of RNase P is a modifier for substrate recognition.

本文言語English
ページ(範囲)94-98
ページ数5
ジャーナルFEBS Letters
491
1-2
DOI
出版ステータスPublished - 2001 2月 23
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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