Hairpin Ribozyme-Mediated Cleavage of the Full-Length β-Glucuronidase (GUS) mRNA

Shin Hisamatsu, Yo Kikuchi*, Shigenori Sonoki

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

We designed three hairpin ribozymes to cleave Escherichia coli β-glucuronidase (GUS) mRNA and tested those activities in vitro. One of the ribozymes was designed to form 9 base pairs in total with the target GUS mRNA, and the other two ribozymes had longer substrate binding sites. All ribozymes cleaved the model substrate (100 bases long) at the predicted target site. Two ribozymes containing longer substrate binding sites cleaved the substrate much more efficiently than the other ribozyme containing shorter substrate binding site. Also, the ribozymes with long substrate binding sites had high activity against the full-length GUS mRNA (1.9 kilobases) and maintained the activity even at a low temperature, 26°C, a general growth condition of plant cells. Effects of the substrate binding site length of the ribozyme on cleavage activity are discussed.

本文言語English
ページ(範囲)294-297
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
59
2
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • バイオテクノロジー
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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