Haploid recombinants formed as sectors in the intraspecific fusants of Aspergillus niger producing citric acid

Kohtaro Kirimura, Sung Pyo Lee, Seiichiro Kawabe, Shoji Usami

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Protoplasts of nutritionally complementary strains of Aspergillus niger producing citric acid were treated in a polyethylene glycol solution, plated onto hypertonic minimal medium and the intraspecific fusants were obtained. When transferred and cultivated on minimal medium, almost all fo the fusants continued to grow as heterokaryons. However, some fusant colonies formed sectors of the prototrophic strains (sector strains). Most of these sector strains were haploid recombinants and their properties were genetically stable when subcultivated on both minimal and supplemented media. With respect of morphological features and citric acid productivity, the recombinant strains were like either of the parental strains or an intermediate between both parental strains. The rest of the sector strains that were nonconidiation typw seemed to be haploid recombinants, although they stopped growing on the third subcultivation on both the minimal and supplemented media.

本文言語English
ページ(範囲)557-562
ページ数6
ジャーナルJournal of Fermentation Technology
65
5
DOI
出版ステータスPublished - 1987

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