hCAS/CSE1L regulates RAD51 distribution and focus formation for homologous recombinational repair

Satoshi Okimoto, Jiying Sun, Atsuhiko Fukuto, Yasunori Horikoshi, Shun Matsuda, Tomonari Matsuda, Masae Ikura, Tsuyoshi Ikura, Shinichi Machida, Hitoshi Kurumizaka, Yoichi Miyamoto, Masahiro Oka, Yoshihiro Yoneda, Yoshiaki Kiuchi, Satoshi Tashiro

    研究成果: Article

    6 引用 (Scopus)

    抜粋

    Homologous recombinational repair (HR) is one of the major repair systems for DNA double-strand breaks. RAD51 is a key molecule in HR, and the RAD51 concentration in the cell nucleus increases after DNA damage induction. However, the mechanism that regulates the intracellular distribution of RAD51 is still unclear. Here, we show that hCAS/CSE1L associates with RAD51 in human cells. We found that hCAS/CSE1L negatively regulates the nuclear protein level of RAD51 under normal conditions. hCAS/CSE1L is also required to repress the DNA damage-induced focus formation of RAD51. Moreover, we show that hCAS/CSE1L plays roles in the regulation of the HR activity and in chromosome stability. These findings suggest that hCAS/CSE1L is responsible for controlling the HR activity by directly interacting with RAD51. We found that hCAS/CSE1L regulates the nuclear concentration of RAD51 and nuclear RAD51 focus formation. hCAS/CSE1L was required to repress the HR activity and to keep chromosome stability. These findings suggest that hCAS/CSE1L is responsible for genome integrity by regulating the intracellular distribution of RAD51.

    元の言語English
    ページ(範囲)681-694
    ページ数14
    ジャーナルGenes to Cells
    20
    発行部数9
    DOI
    出版物ステータスPublished - 2015 9 1

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    フィンガープリント hCAS/CSE1L regulates RAD51 distribution and focus formation for homologous recombinational repair' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Okimoto, S., Sun, J., Fukuto, A., Horikoshi, Y., Matsuda, S., Matsuda, T., Ikura, M., Ikura, T., Machida, S., Kurumizaka, H., Miyamoto, Y., Oka, M., Yoneda, Y., Kiuchi, Y., & Tashiro, S. (2015). hCAS/CSE1L regulates RAD51 distribution and focus formation for homologous recombinational repair. Genes to Cells, 20(9), 681-694. https://doi.org/10.1111/gtc.12262