High-affinity RNA aptamers to C-reactive protein (CRP): Newly developed pre-elution methods for aptamer selection

N. Orito, S. Umekage, K. Sato, S. Kawauchi, H. Tanaka, E. Sakai, T. Tanaka, Yo Kikuchi

研究成果: Article査読

2 被引用数 (Scopus)

抄録

We have developed a modified SELEX (systematic evolution of ligands by exponential enrichment) method to obtain RNA aptamers with high affinity to C-reactive protein (CRP). CRP is a clinical biomarker present in plasma, the level of which increases in response to infections and noninfectious inflammation. The CRP level is also an important prognostic indicator in patients with several syndromes. At present, CRP content in blood is measured immunochemically using antibodies. To develop a more sensitive method using RNA aptamers, we have attempted to obtain high-affinity RNA aptamers to CRP. We succeeded in obtaining an RNA aptamer with high affinity to CRP using a CRP-immobilized Sepharose column and pre-elution procedure. Pre-elution is a method that removes the weak binding portion from a selected RNA population by washing for a short time with buffer containing CRP. By surface plasmon-resonance (SPR) analysis, the affinity constant of this aptamer for CRP was calculated to be K D = 2.25×10 -9 (M). The secondary structure, contact sites with CRP protein, and application of this aptamer will be described.

本文言語English
論文番号012042
ジャーナルJournal of Physics: Conference Series
352
1
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • Physics and Astronomy(all)

フィンガープリント 「High-affinity RNA aptamers to C-reactive protein (CRP): Newly developed pre-elution methods for aptamer selection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル