High-frequency noise attenuation of a two-component system responding to short-pulse input

Akifumi Nishida, Ryoji Sekine, Daisuke Kiga, Masayuki Yamamura

研究成果: Conference contribution

抄録

Among the various biological devices developed and characterized in synthetic biology, light-sensing biological devices can serve as an input-output system owing to their light modulation property. The well-characterized devices in living systems are useful for modulating cellular sensing and transducing information. In this study, we examined short pulse responsiveness of a light-sensing two-component system (TCS), Cph8-OmpR, which was generated by replacing the sensor domain of the EnvZ-OmpR osmoregulatory system with the light sensor Cph1. We varied the input pulse width of the Cph8-OmpR system and found that an input width of <1 s was sufficient to alter the accumulation of a reporter gene upregulated by Cph8 phosphorylation of OmpR. Based on this result and the mathematical model showing that the timescale for the upstream Cph8-activity transition was much faster than that of downstream gene expression, we evaluated the merit of a TCS with such an unbalanced cascade. Our mathematical simulation of a cascade TCS suggests that high-frequency noise arising from fast transitions in kinase activity was attenuated throughout the cascade reaction. In terms of noise attenuation, these results can contribute to analyze biological cascade systems with the balance of reaction rates in each process.

本文言語English
ホスト出版物のタイトルProceedings of the 2016 7th International Conference on Computational Systems-Biology and Bioinformatics, CSBio 2016
出版社Association for Computing Machinery
ページ28-35
ページ数8
ISBN(電子版)9781450347945
DOI
出版ステータスPublished - 2016 12 19
イベント7th International Conference on Computational Systems-Biology and Bioinformatics, CSBio 2016 - Macau, Macao
継続期間: 2016 12 192016 12 22

出版物シリーズ

名前ACM International Conference Proceeding Series

Other

Other7th International Conference on Computational Systems-Biology and Bioinformatics, CSBio 2016
国/地域Macao
CityMacau
Period16/12/1916/12/22

ASJC Scopus subject areas

  • ソフトウェア
  • 人間とコンピュータの相互作用
  • コンピュータ ビジョンおよびパターン認識
  • コンピュータ ネットワークおよび通信

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