High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa; Takafumi Suzuki; Jun Mizuno; Shuichi Shoji; Koji Otsuka

High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa, Takafumi Suzuki, Jun Mizuno, Shuichi Shoji, Koji Otsuka

研究成果: Paper › 査読

抄録

This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

本文言語	English
ページ	362-364
ページ数	3
出版ステータス	Published - 2006 1月 1
イベント	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan 継続期間: 2006 11月 5 → 2006 11月 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
国/地域	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

ASJC Scopus subject areas

化学工学（その他）
バイオエンジニアリング

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引用スタイル

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title = "High performance electrophoretic analysis on cycloolefin polymer microchips",

abstract = "This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.",

keywords = "Chiral separation, Microchip electrophoresis, Protein, Sweeping, Thermal lens microscope",

author = "Fumihiko Kitagawa and Takafumi Suzuki and Jun Mizuno and Shuichi Shoji and Koji Otsuka",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "362--364",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - High performance electrophoretic analysis on cycloolefin polymer microchips

AU - Kitagawa, Fumihiko

AU - Suzuki, Takafumi

AU - Mizuno, Jun

AU - Shoji, Shuichi

AU - Otsuka, Koji

PY - 2006/1/1

Y1 - 2006/1/1

N2 - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

AB - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

KW - Chiral separation

KW - Microchip electrophoresis

KW - Protein

KW - Sweeping

KW - Thermal lens microscope

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M3 - Paper

AN - SCOPUS:84902523691

SP - 362

EP - 364

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

High performance electrophoretic analysis on cycloolefin polymer microchips

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Conference

ASJC Scopus subject areas

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