High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami, Takahiro Arakawa, Sachiko Nomura, Yoshinori Yamaguchi, Shuichi Shoji

研究成果: Paper査読

3 被引用数 (Scopus)

抄録

In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

本文言語English
ページ1774-1776
ページ数3
出版ステータスPublished - 2008
イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
継続期間: 2008 10 122008 10 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域United States
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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