In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.
|出版ステータス||Published - 2008|
|イベント||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States|
継続期間: 2008 10月 12 → 2008 10月 16
|Conference||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008|
|City||San Diego, CA|
|Period||08/10/12 → 08/10/16|
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