Here we report the performance of surface functionalized diamond surfaces as biosensing platform for human immunodeficiency virus transactivator of transcription (HIV-Tat) peptide detection. Comparative investigations were conducted on nanocrystalline diamond (NCD) and polycrystalline diamond (PCD) films. Scanning electron microscopy (SEM) images revealed the morphology differences between NCD and PCD films. X-ray photoelectron spectroscopy (XPS) data showed that functional components and corresponding coverages, demonstrating denser carboxyl acid groups and fluorinated groups on NCD than that PCD films after UV/ozone and fluorine plasma treatment respectively. Contact angle results showed the differences in surface wettability and free energy between functionalized NCD and PCD biosensors. Fluorescence observations confirmed that higher biosensing performance can be obtained on NCD biosensors with high sensitivity selectivity, and stability. The NCD films with denser surface coverages of functionalizations made NCD films much more priority as an effective biosensing candidate than PCD films.
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