High sensitivity in vivo imaging of cancer metastasis using a near-infrared luciferin analogue seMpai

Jun Nakayama*, Ryohei Saito, Yusuke Hayashi, Nobuo Kitada, Shota Tamaki, Yuxuan Han, Kentaro Semba, Shojiro A. Maki

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

本文言語English
論文番号7896
ページ(範囲)1-8
ページ数8
ジャーナルInternational journal of molecular sciences
21
21
DOI
出版ステータスPublished - 2020 11月 1

ASJC Scopus subject areas

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学

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