抄録
Two types of high-speed particle and biomolecule sheath flow sorters were realized using thermosensitive hydrogel. One is all hydrogel sheath flow and the other is two-phase sheath flow that consists of a water flow including samples and two hydrogel carrier flows; these have been utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorting system. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by focused infrared (IR) laser irradiation. High-speed sorting less than 5.0 ms and no error sorting (120 000 counts) in the all hydrogel sheath flow system while 20 ms in the two-phase sheath flow was realized. Since sorting was performed in simple PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.
本文言語 | English |
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論文番号 | S04 |
ページ(範囲) | 3141-3146 |
ページ数 | 6 |
ジャーナル | Measurement Science and Technology |
巻 | 17 |
号 | 12 |
DOI | |
出版ステータス | Published - 2006 12月 1 |
ASJC Scopus subject areas
- 器械工学
- 工学(その他)
- 応用数学