High-throughput processing of proteins using a porous and tentacle anion-exchange membrane

Satoshi Tsuneda, Kyoichi Saito*, Shintaro Furusaki, Takanobu Sugo

*この研究の対応する著者

研究成果: Article査読

103 被引用数 (Scopus)

抄録

The immobilization of polymer chains containing a diethylamino (DEA) group on the pore surface of a porous hollow-fibre membrane is reported. This novel membrane can collect proteins at a high rate and high capacity because of convective transport and multi-layering of proteins. Overlapping of the breakthrough curves for different residence times of bovine serum albumin (BSA) solution demonstrates that the diffusional resistance of BSA to the DEA group anchored to the polymer chain was negligible. Membranes with a higher density of DEA groups exhibited a higher binding capacity for BSA. For example, a membrane with a DEA group density of 2.9 mol/kg had a BSA binding capacity of 490 g/kg, which was equivalent to eleven times the adsorption capacity of a monolayer. This vertical layering is due to holding of the BSA molecules in a tentacle-like manner by the graft chains extending from the pore surface towards the pore interior.

本文言語English
ページ(範囲)211-218
ページ数8
ジャーナルJournal of Chromatography A
689
2
DOI
出版ステータスPublished - 1995 1 13
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学

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