抄録
The immobilization of polymer chains containing a diethylamino (DEA) group on the pore surface of a porous hollow-fibre membrane is reported. This novel membrane can collect proteins at a high rate and high capacity because of convective transport and multi-layering of proteins. Overlapping of the breakthrough curves for different residence times of bovine serum albumin (BSA) solution demonstrates that the diffusional resistance of BSA to the DEA group anchored to the polymer chain was negligible. Membranes with a higher density of DEA groups exhibited a higher binding capacity for BSA. For example, a membrane with a DEA group density of 2.9 mol/kg had a BSA binding capacity of 490 g/kg, which was equivalent to eleven times the adsorption capacity of a monolayer. This vertical layering is due to holding of the BSA molecules in a tentacle-like manner by the graft chains extending from the pore surface towards the pore interior.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 211-218 |
| ページ数 | 8 |
| ジャーナル | Journal of Chromatography A |
| 巻 | 689 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 1995 1 13 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Organic Chemistry
これを引用
High-throughput processing of proteins using a porous and tentacle anion-exchange membrane. / Tsuneda, Satoshi; Saito, Kyoichi; Furusaki, Shintaro; Sugo, Takanobu.
：: Journal of Chromatography A, 巻 689, 番号 2, 13.01.1995, p. 211-218.研究成果: Article
}
TY - JOUR
T1 - High-throughput processing of proteins using a porous and tentacle anion-exchange membrane
AU - Tsuneda, Satoshi
AU - Saito, Kyoichi
AU - Furusaki, Shintaro
AU - Sugo, Takanobu
PY - 1995/1/13
Y1 - 1995/1/13
N2 - The immobilization of polymer chains containing a diethylamino (DEA) group on the pore surface of a porous hollow-fibre membrane is reported. This novel membrane can collect proteins at a high rate and high capacity because of convective transport and multi-layering of proteins. Overlapping of the breakthrough curves for different residence times of bovine serum albumin (BSA) solution demonstrates that the diffusional resistance of BSA to the DEA group anchored to the polymer chain was negligible. Membranes with a higher density of DEA groups exhibited a higher binding capacity for BSA. For example, a membrane with a DEA group density of 2.9 mol/kg had a BSA binding capacity of 490 g/kg, which was equivalent to eleven times the adsorption capacity of a monolayer. This vertical layering is due to holding of the BSA molecules in a tentacle-like manner by the graft chains extending from the pore surface towards the pore interior.
AB - The immobilization of polymer chains containing a diethylamino (DEA) group on the pore surface of a porous hollow-fibre membrane is reported. This novel membrane can collect proteins at a high rate and high capacity because of convective transport and multi-layering of proteins. Overlapping of the breakthrough curves for different residence times of bovine serum albumin (BSA) solution demonstrates that the diffusional resistance of BSA to the DEA group anchored to the polymer chain was negligible. Membranes with a higher density of DEA groups exhibited a higher binding capacity for BSA. For example, a membrane with a DEA group density of 2.9 mol/kg had a BSA binding capacity of 490 g/kg, which was equivalent to eleven times the adsorption capacity of a monolayer. This vertical layering is due to holding of the BSA molecules in a tentacle-like manner by the graft chains extending from the pore surface towards the pore interior.
UR - http://www.scopus.com/inward/record.url?scp=0028816757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028816757&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(94)00911-R
DO - 10.1016/0021-9673(94)00911-R
M3 - Article
AN - SCOPUS:0028816757
VL - 689
SP - 211
EP - 218
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 2
ER -