High-throughput pyrosequencing of the chloroplast genome of a highly neutral-lipid-producing marine pennate diatom, Fistulifera sp. strain JPCC DA0580

Tsuyoshi Tanaka*, Yorikane Fukuda, Tomoko Yoshino, Yoshiaki Maeda, Masaki Muto, Mitsufumi Matsumoto, Shigeki Mayama, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

31 被引用数 (Scopus)

抄録

The chloroplast genome of the highly neutrallipid- producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp with an inverted repeat of 13,330 bp and is slightly larger than the other diatom chloroplast genomes due to several low genedensity regions lacking similarity to the other diatom chloroplast genomes. Protein-coding genes were nearly identical to those from Phaeodactylum tricornutum. On the other hand, we found unique sequence variations in genes of photosystem II which differ from the consensus in other diatom chloroplasts. Furthermore, five functional unknown ORFs and a putative serine recombinase gene, serC2, are located in the low gene-density regions. SerC2 was also identified in the plasmids of another pennate diatom, Cylindrotheca fusiformis, and in the plastid genome of the diatom endosymbiont of Kryptoperidinium foliaceum. Exogenous plasmids might have been incorporated into the chloroplast genome of Fistulifera sp. by lateral gene transfer. Chloroplast genome sequencing analysis of this novel diatom provides many important insights into diatom evolution.

本文言語English
ページ(範囲)223-229
ページ数7
ジャーナルPhotosynthesis Research
109
1-3
DOI
出版ステータスPublished - 2011 9月
外部発表はい

ASJC Scopus subject areas

  • 植物科学
  • 細胞生物学
  • 生化学

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