High-vacuum vapor deposition and in situ monitoring of N-carboxy anhydride benzyl glutamate polymerization

Hatice Duran, Kenta Ogura, Kenji Nakao, Sulivan D B Vianna, Hiroaki Usui*, Rigoberto C. Advincula, Wolfgang Knoll

*この研究の対応する著者

研究成果: Article査読

21 被引用数 (Scopus)

抄録

A high-vacuum physical vapor deposition (PVD) method was used to grow polypeptide chains from gold surfaces coupled with in situ monitoring using surface plasmon resonance spectroscopy (SPR). Polymerization of N-carboxy anhydride of benzyl-L-glutamate (BLG-NCA) was demonstrated, forming grafted polybenzyl-L-glutamate (PBLG) films. 2-Aminoethanethiol (AET) was used as the initiating site either deposited as a self-assembled monolayer (SAM), as a PVD film, or codeposited on the gold substrates. PBLG films up to 30 nm thick in which the formation of different secondary structures was monitored by in situ SPR and ex situ Fourier transform infrared spectroscopy (FT-IR) methods based on the parameters for deposition were prepared. Furthermore, surface topology and film morphology properties were determined on the basis of AFM measurements and were found to depend largely on the deposition process of the AET. Besides investigation of the AET surface density effects, the orientation of the PBLG chains was also characterized using ex situ FT-IR.

本文言語English
ページ(範囲)10711-10718
ページ数8
ジャーナルLangmuir
25
18
DOI
出版ステータスPublished - 2009 9 15
外部発表はい

ASJC Scopus subject areas

  • 電気化学
  • 凝縮系物理学
  • 表面および界面
  • 材料科学(全般)
  • 分光学

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