Highly sensitive detection of allergen using bacterial magnetic particles

N. Nakamura*, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

68 被引用数 (Scopus)

抄録

A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.

本文言語English
ページ(範囲)585-589
ページ数5
ジャーナルAnalytica Chimica Acta
281
3
DOI
出版ステータスPublished - 1993 9 24
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分析化学
  • 分光学
  • 環境化学

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