Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

A. Rahim Ruslinda*, Varun Penmatsa, Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

*この研究の対応する著者

研究成果: Article査読

42 被引用数 (Scopus)

抄録

Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl2 buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg2+ concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.

本文言語English
ページ(範囲)1692-1697
ページ数6
ジャーナルAnalyst
137
7
DOI
出版ステータスPublished - 2012 4月 7

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学
  • 電気化学

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