Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

A. Rahim Ruslinda, Varun Penmatsa, Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

    研究成果: Article

    36 引用 (Scopus)

    抄録

    Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl 2 buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg 2+ concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.

    元の言語English
    ページ(範囲)1692-1697
    ページ数6
    ジャーナルAnalyst
    137
    発行部数7
    DOI
    出版物ステータスPublished - 2012 4 7

    Fingerprint

    Diamond
    Platelet-Derived Growth Factor
    Platelets
    diamond
    Diamonds
    Fluorescence
    fluorescence
    Biomolecules
    Ionic strength
    Osmolar Concentration
    Limit of Detection
    Buffers
    Binding sites
    Binding Sites
    Intercellular Signaling Peptides and Proteins
    detection

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Spectroscopy
    • Electrochemistry
    • Biochemistry
    • Environmental Chemistry

    これを引用

    Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design. / Ruslinda, A. Rahim; Penmatsa, Varun; Ishii, Yoko; Tajima, Shinya; Kawarada, Hiroshi.

    :: Analyst, 巻 137, 番号 7, 07.04.2012, p. 1692-1697.

    研究成果: Article

    Ruslinda, A. Rahim ; Penmatsa, Varun ; Ishii, Yoko ; Tajima, Shinya ; Kawarada, Hiroshi. / Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design. :: Analyst. 2012 ; 巻 137, 番号 7. pp. 1692-1697.
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    AU - Kawarada, Hiroshi

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