Highly sensitive real-time PCR assay for quantification of toxic cyanobacteria based on microcystin synthetase a gene

Kazuhiro Furukawa, Naohiro Noda*, Satoshi Tsuneda, Takeshi Saito, Tomoaki Itayama, Yuhei Inamori

*この研究の対応する著者

研究成果: Article査読

52 被引用数 (Scopus)

抄録

The presence of cyanobacterial bloom in water supply reservoirs can cause potential health hazards. In this study, we aimed at the quantification of microcystin-producing cyanobacteria based on the microcystin synthetase A (mcyA) gene using real-time PCR. To perform a highly sensitive real-time PCR assay, the novel primer MSR-2R was designed and a coprecipitation DNA extraction method was used in this study. Cyanobacterial cells could be collected efficiently by coprecipitation with other bacteria suspended in solution even in the case of low concentrations of cyanobacteria. The detection limit of the method was found to be 8.8 cells per reaction. When cyanobacterial growth was monitored in pure culture, the cell concentration determined by real-time PCR positively correlated with the cell concentration determined from direct microscopic count. Furthermore, we could detect and quantify the mcyA gene in lake water samples using real-time PCR. It was concluded that the quantification of the mcyA gene based on real-time PCR is a powerful tool for the rapid quantification of microcystin-producing cyanobacteria in environmental samples.

本文言語English
ページ(範囲)90-96
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
102
2
DOI
出版ステータスPublished - 2006 8

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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