TY - JOUR
T1 - Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors
AU - Tseeb, Vadim
AU - Suzuki, Madoka
AU - Oyama, Kotaro
AU - Iwai, Kaoru
AU - Ishiwata, Shin'ichi
PY - 2009/4
Y1 - 2009/4
N2 - Intracellular Ca 2+ distribution and its dynamics are essential for various cellular functions. We show with single HeLa cells that a microscopic heat pulse induces Ca 2+ uptake into intracellular stores during heating and Ca 2+ release from them at the onset of recooling, and the overshoot of Ca 2+ release occurs above the critical value of a temperature change, which decreases from 1.5 to 0.2 °C on increasing the experimental temperature from 22 to 37 °C. This highly thermosensitive Ca 2+ dynamics is probably attributable to the altered balance between Ca 2+ uptake by endoplasmic reticulum Ca 2+-ATPases and Ca 2+ release via inositol 1,4,5-trisphosphate receptors. These results suggest that Ca 2+ signaling is extremely sensitive to temperature changes, especially around body temperature, in cells expressing inositol 1,4,5-trisphosphate receptors.
AB - Intracellular Ca 2+ distribution and its dynamics are essential for various cellular functions. We show with single HeLa cells that a microscopic heat pulse induces Ca 2+ uptake into intracellular stores during heating and Ca 2+ release from them at the onset of recooling, and the overshoot of Ca 2+ release occurs above the critical value of a temperature change, which decreases from 1.5 to 0.2 °C on increasing the experimental temperature from 22 to 37 °C. This highly thermosensitive Ca 2+ dynamics is probably attributable to the altered balance between Ca 2+ uptake by endoplasmic reticulum Ca 2+-ATPases and Ca 2+ release via inositol 1,4,5-trisphosphate receptors. These results suggest that Ca 2+ signaling is extremely sensitive to temperature changes, especially around body temperature, in cells expressing inositol 1,4,5-trisphosphate receptors.
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U2 - 10.2976/1.3073779
DO - 10.2976/1.3073779
M3 - Article
C2 - 19597558
AN - SCOPUS:77956342305
VL - 3
SP - 117
EP - 123
JO - Frontiers in Life Science
JF - Frontiers in Life Science
SN - 2155-3769
IS - 2
ER -