HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment

Hiroaki Tachiwana, Sebastian Müller, Julia Blümer, Kerstin Klare, Andrea Musacchio, Geneviève Almouzni

研究成果: Article

39 引用 (Scopus)

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Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3<sup>CENP-A</sup> in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3<sup>CENP-A</sup> contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores. We find that de novo CENP-T accumulation depends on CENP-C and that recruitment of these factors requires two domains in CenH3<sup>CENP-A</sup>: the HJURP-binding region (CATD) and the CENP-C-binding region (CAC). Notably, HJURP interacts directly with CENP-C and is critical for de novo accumulation of CENP-C at synthetic centromeres. On the basis of our findings, we propose that HJURP serves a dual chaperone function in coordinating CenH3<sup>CENP-A</sup> and CENP-C recruitment.

元の言語English
ページ(範囲)22-32
ページ数11
ジャーナルCell Reports
11
発行部数1
DOI
出版物ステータスPublished - 2015 4 7
外部発表Yes

    フィンガープリント

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Tachiwana, H., Müller, S., Blümer, J., Klare, K., Musacchio, A., & Almouzni, G. (2015). HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment. Cell Reports, 11(1), 22-32. https://doi.org/10.1016/j.celrep.2015.03.013