Holliday junction-binding activity of human SPF45

Naoki Horikoshi, Yuichi Morozumi, Motoki Takaku, Yoshimasa Takizawa, Hitoshi Kurumizaka

研究成果: Article

5 引用 (Scopus)

抄録

SPF45 is considered to be a bifunctional protein that functions in splicing and DNA repair. A previous genetic study reported that Drosophila SPF45 participates in the DNA-repair pathway with a RAD51-family protein, RAD201, suggesting that SPF45 may function in DNA repair by the homologous-recombination pathway. To study the function of SPF45 in homologous recombination, we purified human SPF45 and found that it preferentially binds to the Holliday junction, which is a key DNA intermediate in the homologous-recombination pathway. Deletion analyses revealed that the RNA recognition motif, which is located in the C-terminal region of human SPF45, is not involved in DNA binding. On the other hand, alanine-scanning mutagenesis identified the N-terminal lysine residues, which may be involved in Holliday junction binding by human SPF45. We also found that human SPF45 significantly binds to a RAD51 paralog, RAD51B, although it also binds to RAD51 and DMC1 with lower affinity. These biochemical results support the idea that human SPF45 functions in DNA repair by homologous recombination.

元の言語English
ページ(範囲)373-383
ページ数11
ジャーナルGenes to Cells
15
発行部数4
DOI
出版物ステータスPublished - 2010 4

Fingerprint

Cruciform DNA
Human Activities
Recombinational DNA Repair
Homologous Recombination
DNA Repair
Rad51 Recombinase
DNA
Mutagenesis
Alanine
Lysine
Drosophila
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Medicine(all)

これを引用

Horikoshi, N., Morozumi, Y., Takaku, M., Takizawa, Y., & Kurumizaka, H. (2010). Holliday junction-binding activity of human SPF45. Genes to Cells, 15(4), 373-383. https://doi.org/10.1111/j.1365-2443.2010.01383.x

Holliday junction-binding activity of human SPF45. / Horikoshi, Naoki; Morozumi, Yuichi; Takaku, Motoki; Takizawa, Yoshimasa; Kurumizaka, Hitoshi.

:: Genes to Cells, 巻 15, 番号 4, 04.2010, p. 373-383.

研究成果: Article

Horikoshi, N, Morozumi, Y, Takaku, M, Takizawa, Y & Kurumizaka, H 2010, 'Holliday junction-binding activity of human SPF45', Genes to Cells, 巻. 15, 番号 4, pp. 373-383. https://doi.org/10.1111/j.1365-2443.2010.01383.x
Horikoshi N, Morozumi Y, Takaku M, Takizawa Y, Kurumizaka H. Holliday junction-binding activity of human SPF45. Genes to Cells. 2010 4;15(4):373-383. https://doi.org/10.1111/j.1365-2443.2010.01383.x
Horikoshi, Naoki ; Morozumi, Yuichi ; Takaku, Motoki ; Takizawa, Yoshimasa ; Kurumizaka, Hitoshi. / Holliday junction-binding activity of human SPF45. :: Genes to Cells. 2010 ; 巻 15, 番号 4. pp. 373-383.
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