Holliday junction binding activity of the human Rad51B protein

Hiroshi Yokoyama, Hitoshi Kurumizaka, Shukuko Ikawa, Shigeyuki Yokoyama*, Takehiko Shibata

*この研究の対応する著者

研究成果: Article査読

52 被引用数 (Scopus)

抄録

The human Rad51B protein is involved in the recombinational repair of damaged DNA. Chromosomal rearrangements of the Rad51B gene have been found in uterine leiomyoma patients, suggesting that the Rad51B gene suppresses tumorigenesis. In the present study, we found that the purified Rad51B protein bound to single-stranded DNA and double-stranded DNA in the presence of ATP and either Mg2+ or Mn2+ and hydrolyzed ATP in a DNA-dependent manner. When the synthetic Holliday junction was present along with the half-cruciform and double-stranded oligonucleotides, the Rad51B protein only bound to the synthetic Holliday junction, which mimics a key intermediate in homologous recombination. In contrast, the human Rad51 protein bound to all three DNA substrates with no obvious preference. Therefore, the Rad51B protein may have a specific function in Holliday junction processing in the homologous recombinational repair pathway in humans.

本文言語English
ページ(範囲)2767-2772
ページ数6
ジャーナルJournal of Biological Chemistry
278
4
DOI
出版ステータスPublished - 2003 1 24
外部発表はい

ASJC Scopus subject areas

  • 生化学

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