Homologous genetic recombination as an intrinsic dynamic property of a DNA structure induced by recA/rad51-family proteins: A possible advantage of DNA over RNA as genomic material

Takehiko Shibata*, Taro Nishinaka, Tsutomu Mikawa, Hideki Aihara, Hitoshi Kurumizaka, Shigeyuki Yokoyama, Yutaka Ito

*この研究の対応する著者

研究成果: Article査読

58 被引用数 (Scopus)

抄録

Heteroduplex joints are general intermediates of homologous genetic recombination in DNA genomes. A heteroduplex joint is formed between a single-stranded region (or tail), derived from a cleaved parental double-stranded DNA, and homologous regions in another parental double-stranded DNA, in a reaction mediated by the RecA/Rad51-family of proteins. In this reaction, a RecA/Rad51-family protein first forms a filamentous complex with the single-stranded DNA, and then interacts with the double-stranded DNA in a search for homology. Studies of the three-dimensional structures of single-stranded DNA bound either to Escherichia coli RecA or Saccharomyces cerevisiae Rad51 have revealed a novel extended DNA structure. This structure contains a hydrophobic interaction between the 2′ methylene moiety of each deoxyribose and the aromatic ring of the following base, which allows bases to rotate horizontally through the interconversion of sugar puckers. This base rotation explains the mechanism of the homology search and base-pair switch between double-stranded and single-stranded DNA during the formation of heteroduplex joints. The pivotal role of the 2′ methylene-base interaction in the heteroduplex joint formation is supported by comparing the recombination of RNA genomes with that of DNA genomes. Some simple organisms with DNA genomes induce homologous recombination when they encounter conditions that are unfavorable for their survival. The extended DNA structure confers a dynamic property on the otherwise chemically and genetically stable double-stranded DNA, enabling gene segment rearrangements without disturbing the coding frame (i.e., protein-segment shuffling). These properties may give an extensive evolutionary advantage to DNA.

本文言語English
ページ(範囲)8425-8432
ページ数8
ジャーナルProceedings of the National Academy of Sciences of the United States of America
98
15
DOI
出版ステータスPublished - 2001 7月 17
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 一般

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