Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1

Wataru Kobayashi, Enwei Liu, Hajime Ishii, Sachihiro Matsunaga, Peter Schlögelhofer, Hitoshi Kurumizaka

    研究成果査読

    2 被引用数 (Scopus)

    抄録

    In eukaryotes, homologous recombination plays a pivotal role in both genome maintenance and generation of genetic diversity. Eukaryotic RecA homologues, RAD51 and DMC1, are key proteins in homologous recombination that promote pairing between homologous DNA sequences. Arabidopsis thaliana is a prominent model plant for studying eukaryotic homologous recombination. However, A. thaliana RAD51 and DMC1 have not been biochemically characterized. In the present study, we purified A. thaliana RAD51 (AtRAD51) and DMC1 (AtDMC1). Biochemical analyses revealed that both AtRAD51 and AtDMC1 possess ATP hydrolyzing activity, filament formation activity and homologous pairing activity in vitro. We then compared the homologous pairing activities of AtRAD51 and AtDMC1 with those of the Oryza sativa and Homo sapiens RAD51 and DMC1 proteins.

    本文言語English
    ページ(範囲)289-295
    ページ数7
    ジャーナルJournal of biochemistry
    165
    3
    DOI
    出版ステータスPublished - 2019 3 1

    ASJC Scopus subject areas

    • 生化学
    • 分子生物学

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