Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex

Hitoshi Kurumizaka, Shukuko Ikawa, Maki Nakada, Rima Enomoto, Wataru Kagawa, Takashi Kinebuchi, Mitsuyoshi Yamazoe, Shigeyuki Yokoyama, Takehiko Shibata*

*この研究の対応する著者

研究成果: Article査読

64 被引用数 (Scopus)

抄録

The Xrcc2 and Rad51D/Rad51L3 proteins, which belong to the Rad51 paralogs, are required for homologous recombinational repair (HRR) in vertebrates. The Xrcc2 and Rad51D/Rad51L3 genes, whose products interact with each other, have essential roles in ensuring normal embryonic development. In the present study, we coexpressed the human Xrcc2 and Rad51D/Rad51L3 proteins (Xrcc2 and Rad51D, respectively) in Escherichia coli, and purified the Xrcc2·Rad51D complex to homogeneity. The Xrcc2·Rad51D complex catalyzed homologous pairing between single-stranded and double-stranded DNA, similar to the function of the Xrcc3· Rad51C complex, which is another complex of the Rad51 paralogs. An electron microscopic analysis showed that Xrcc2·Rad51D formed a multimeric ring structure in the absence of DNA. In the presence of ssDNA, Xrcc2·Rad-51D formed a filamentous structure, which is commonly observed among the human homologous pairing proteins, Rad51, Rad52, and Xrcc3·Rad51C.

本文言語English
ページ(範囲)14315-14320
ページ数6
ジャーナルJournal of Biological Chemistry
277
16
DOI
出版ステータスPublished - 2002 4月 19
外部発表はい

ASJC Scopus subject areas

  • 生化学

フィンガープリント

「Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル