Hypertrophy of rat skeletal muscle is associated with increased sirt1/akt/mtor/s6 and suppressed sestrin2/sirt3/foxo1 levels

Zoltan Gombos, Erika Koltai, Ferenc Torma, Peter Bakonyi, Attila Kolonics, Dora Aczel, Tamas Ditroi, Peter Nagy, Takuji Kawamura, Zsolt Radak*

*この研究の対応する著者

研究成果: Article査読

抄録

Despite the intensive investigation of the molecular mechanism of skeletal muscle hypertrophy, the underlying signaling processes are not completely understood. Therefore, we used an overload model, in which the main synergist muscles (gastrocnemius, soleus) of the plantaris muscle were surgically removed, to cause a significant overload in the remaining plantaris muscle of 8‐ month‐old Wistar male rats. SIRT1‐associated pro‐anabolic, pro‐catabolic molecular signaling pathways, NAD and H2S levels of this overload‐induced hypertrophy were studied. Fourteen days of overload resulted in a significant 43% (p < 0.01) increase in the mass of plantaris muscle compared to sham operated animals. Cystathionine‐β‐synthase (CBS) activities and bioavailable H2S levels were not modified by overload. On the other hand, overload‐induced hypertrophy of skeletal muscle was associated with increased SIRT1 (p < 0.01), Akt (p < 0.01), mTOR, S6 (p < 0.01) and suppressed sestrin 2 levels (p < 0.01), which are mostly responsible for anabolic signaling. Decreased FOXO1 and SIRT3 signaling (p < 0.01) suggest downregulation of protein breakdown and mitophagy. Decreased levels of NAD+, sestrin2, OGG1 (p < 0.01) indicate that the redox milieu of skeletal muscle after 14 days of overloading is reduced. The present investigation revealed novel cellular interactions that regulate anabolic and catabolic processes in the hypertrophy of skeletal muscle.

本文言語English
論文番号7588
ジャーナルInternational journal of molecular sciences
22
14
DOI
出版ステータスPublished - 2021 7 2

ASJC Scopus subject areas

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学

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