Identification and characterization of key substructures involved in the early folding events of a (β/α)8-barrel protein as studied by experimental and computational methods

Satoshi Akanuma, Akihiko Yamagishi

研究成果: Article査読

13 被引用数 (Scopus)

抄録

A number of studies have examined the structural properties of late folding intermediates of (β/α)8-barrel proteins involved in tryptophan biosynthesis, whereas there is little information available about the early folding events of these proteins. To identify the contiguous polypeptide segments important to the folding of the (β/α)8-barrel protein Escherichia coli N-(5′-phosphoribosyl)anthranilate isomerase, we structurally characterized fragments and circularly permuted forms of the protein. We also simulated thermal unfolding of the protein using molecular dynamics. Our fragmentation experiments demonstrate that the isolated (β/α)1-4β5 fragment is almost as stable as the full-length protein. The far and near-UV CD spectra of this fragment are indicative of native-like secondary and tertiary structures. Structural analysis of the circularly permutated proteins shows that if the protein is cleaved within the two N-terminal βα modules, the amount of secondary structure is unaffected, whereas, when cleaved within the central (β/α)3-4β5 segment, the protein simply cannot fold. An ensemble of the denatured structures produced by thermal unfolding simulations contains a persistent local structure comprised of β3, β4 and β5. The presence of this three-stranded β-barrel suggests that it may be an important early-stage folding intermediate. Interactions found in (β/α) 3-4β5 may be essential for the early events of ePRAI folding if they provide a nucleation site that directs folding.

本文言語English
ページ(範囲)1161-1170
ページ数10
ジャーナルJournal of Molecular Biology
353
5
DOI
出版ステータスPublished - 2005 11 11
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 分子生物学

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