TY - JOUR
T1 - Identification, localization, and function of a novel avian hypothalamic neuropeptide, 26RFa, and its cognate receptor, G protein-coupled receptor-103
AU - Ukena, Kazuyoshi
AU - Tachibana, Tetsuya
AU - Iwakoshi-Ukena, Eiko
AU - Saito, Yumiko
AU - Minakata, Hiroyuki
AU - Kawaguchi, Ryoko
AU - Osugi, Tomohiro
AU - Tobari, Yasuko
AU - Leprince, Jérôme
AU - Vaudry, Hubert
AU - Tsutsui, Kazuyoshi
PY - 2010/5
Y1 - 2010/5
N2 - Several neuropeptides with the C-terminal RFamide sequence have been identified in the hypothalamus of a variety of vertebrates. Among the RFamide peptide groups, however, only LPXRFamide peptides, including gonadotropin-inhibitory hormone, have been characterized in the avian brain. In the present study, we sought for the presence of other RFamide peptides in the avian hypothalamus. We identified a cDNA encoding an RFamide peptide orthologous to 26RFa (also referred to as QRFP) in thehypothalamusof the Japanese quail.Thededucedquail26RFaprecursor consisted of 120-amino-acid residues, encoding one RFamide peptide with 27 amino acids. This RFamide peptide was flanked at the N terminus by a dibasic amino acid cleavage site and at the C terminus by a glycine amidation signal. Quantitative RT-PCR analysis demonstrated specific expression of quail 26RFa mRNA in the diencephalon including the hypothalamus. Furthermore, mass spectrometry analysis revealed the presence of a peptide exhibiting the mass of mature 26RFa, indicating that the peptide is actually produced from the precursor in the diencephalon. 26RFa-producing cell bodies were localized in the anterior hypothalamic nucleus in the brain. Synthetic 26RFa increased intracellular Ca2+ concentration in HEK293T cells transfected with the chicken G protein-coupled receptor GPR103. Intracerebro-ventricular injection of 26RFa in broiler chicks stimulated feeding behavior. These data provide the first evidence for the occurrence of the peptide 26RFa in the avian hypothalamus and indicate that this peptide exerts orexigenic activity.
AB - Several neuropeptides with the C-terminal RFamide sequence have been identified in the hypothalamus of a variety of vertebrates. Among the RFamide peptide groups, however, only LPXRFamide peptides, including gonadotropin-inhibitory hormone, have been characterized in the avian brain. In the present study, we sought for the presence of other RFamide peptides in the avian hypothalamus. We identified a cDNA encoding an RFamide peptide orthologous to 26RFa (also referred to as QRFP) in thehypothalamusof the Japanese quail.Thededucedquail26RFaprecursor consisted of 120-amino-acid residues, encoding one RFamide peptide with 27 amino acids. This RFamide peptide was flanked at the N terminus by a dibasic amino acid cleavage site and at the C terminus by a glycine amidation signal. Quantitative RT-PCR analysis demonstrated specific expression of quail 26RFa mRNA in the diencephalon including the hypothalamus. Furthermore, mass spectrometry analysis revealed the presence of a peptide exhibiting the mass of mature 26RFa, indicating that the peptide is actually produced from the precursor in the diencephalon. 26RFa-producing cell bodies were localized in the anterior hypothalamic nucleus in the brain. Synthetic 26RFa increased intracellular Ca2+ concentration in HEK293T cells transfected with the chicken G protein-coupled receptor GPR103. Intracerebro-ventricular injection of 26RFa in broiler chicks stimulated feeding behavior. These data provide the first evidence for the occurrence of the peptide 26RFa in the avian hypothalamus and indicate that this peptide exerts orexigenic activity.
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U2 - 10.1210/en.2009-1478
DO - 10.1210/en.2009-1478
M3 - Article
C2 - 20308530
AN - SCOPUS:77953159608
VL - 151
SP - 2255
EP - 2264
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 5
ER -