Identification of a novel Sry-related gene and its germ cell-specific expression

Emiko Osaki, Yukio Nishina, Johji Inazawa, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Miho Ohsugi, Tohru Tezuka, Mitsuaki Yoshida, Kentaro Semba*

*この研究の対応する著者

研究成果: Article査読

75 被引用数 (Scopus)

抄録

Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-determining factor. At present almost 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development, Some are involved in human diseases, including sex reversal. Here we report the isolation of a novel member of the Sox gene family, Sox30, which may constitute a distinct subgroup of this family. Using a bacterially expressed DNA-binding domain of Sox30, we show that it is able to specifically recognize the ACAAT motif. Furthermore, Sox30 is capable of activating transcription from a synthetic promoter containing the ACAAT motif. The specific expression of Sox30 in normal testes, but not in maturing germ cell-deficient testes, suggests the involvement of Sox30 in differentiation of male germ cells. Mapping analyses revealed that the Sox30 gene is located on human chromosome 5 (5q33) and on mouse chromosome 11.

本文言語English
ページ(範囲)2503-2510
ページ数8
ジャーナルNucleic acids research
27
12
DOI
出版ステータスPublished - 1999 6月 15
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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