Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis

Takuya Chiba*, Junjie Yao, Yoshikazu Higami, Isao Shimokawa, Masanori Hosokawa, Keiichi Higuchi

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Senescence-accelerated mouse (SAM) strains constitute a model of accelerated senescence coupled with a short lifespan and the early development of various age-related disorders. To identify differential gene expression in testes between senescence-accelerated SAMP1 and control SAMR1 mice, we performed suppression subtractive hybridization. We observed that the expression of three genes related to cell proliferation (myosin regulatory light chain B, aldolase 1A isoform, and cytochrome c oxidase subunit VIc) were upregulated and four genes implicated in spermatogenesis were downregulated in SAMP1 mice. Asb-8, a member of ankyrin repeat-containing proteins, was abundantly expressed in the testes and downregulated in SAMP1. The other three downregulated genes (germ cell-specific gene 1, T-complex polypeptide 1b, and activator of cAMP responsive element modulator in testis) have been reported to regulate late-stage spermatogenesis. These gene expression profiles might explain the findings of early testicular maturation and rapid decline in the ability to produce spermatozoa with advancing age in SAMP1 mice.

本文言語English
ページ(範囲)105-112
ページ数8
ジャーナルMammalian Genome
18
2
DOI
出版ステータスPublished - 2007 2
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル