Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis

Nami Nogawa-Kosaka, Tatsuhisa Sugai, Kazumichi Nagasawa, Yuta Tanizaki, Mizue Meguro, Youichi Aizawa, Shun Maekawa, Motoyasu Adachi, Ryota Kuroki, Takashi Kato*

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Oxygen is essential for the survival of animals. Red blood cells in the circulation, i.e. peripheral erythrocytes, are responsible for transporting oxygen to tissues. The regulation of erythropoiesis in vertebrates other than mammals is yet to be elucidated. Recently we identified erythropoietin, a primary regulator of erythropoiesis, in Xenopus laevis, which should enable us to identify target cells, including erythroid progenitors, and to investigate the production and development of erythroid cells in amphibians. Here, we established a semi-solid colony-forming assay in Xenopus laevis to clarify the existence of colony-forming unit-erythroid cells, the functional erythroid progenitors identified in vitro. Using this assay, we showed that recombinant xlEPO induces erythroid colony formation in vitro and detected an increased level of erythropoietin activity in blood serum during acute anemic stress. In addition, our study demonstrated the possible presence of multiple, non-x/EPO, factors in anemic serum supportive of erythroid colony formation. These results indicate that erythropoiesis mediated by erythropoietin is present in amphibian species and, furthermore, that the regulatory mechanisms controlling peripheral erythrocyte number may vary among vertebrates.

本文言語English
ページ(範囲)921-927
ページ数7
ジャーナルJournal of Experimental Biology
214
6
DOI
出版ステータスPublished - 2011 3月

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 生理学
  • 水圏科学
  • 動物科学および動物学
  • 分子生物学
  • 昆虫科学

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